Journal: Journal of Animal Science
Article Title: Manipulating early-life environment and handling alters growth trajectories, immune and stress responses, clinical indicators, and intestinal health in young pigs
doi: 10.1093/jas/skaf343
Figure Lengend Snippet: Secretory IgA concentration within the feces of pigs collected on PND 21 or 27. Pigs were artificially- or sow-reared, and daily- or weekly-handled from PND 2-21. Abbreviations: AR-D, artificially-reared, daily-handled; AR-W, artificially-reared, weekly-handled; H, handling; IgA, immunoglobulin A; ME, main effect; PND, postnatal day; R, rearing; SR-D, sow-reared, daily-handled; SR-W, sow-reared, weekly-handled. Main effect is denoted with bar separation, with the P -value displayed above.
Article Snippet: Subsequently, the sample tubes were homogenized (TissueLyser II, Qiagen, Valencia, CA) at 30 Hz for 15 sec before being centrifuged for 15 min at 12,000 × g. The upper aqueous layer was collected and utilized to quantify secretory IgA concentrations via a sandwich ELISA (catalog # PGA51-01; Eagle biosciences; Nashua, NH).
Techniques: Concentration Assay